Kohls, Sarah and Scholz-Böttcher, Barbara and Rullkötter, Jürgen and Teske, Jörg (2012) Method validation of a survey of thevetia cardiac glycosides in serum samples. Forensic Science International, 215. pp. 146-151. ISSN 0379-0738

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Abstract

A sensitive and specific liquid chromatography tandem mass spectrometry (HPLC–ESI+-MS/MS) procedure was developed and validated for the identification and quantification of thevetin B and further cardiac glycosides in human serum. The seeds of Yellow Oleander (Thevetia peruviana) contain cardiac glycosides that can cause serious intoxication. A mixture of six thevetia glycosides was extracted from these seeds and characterized. Thevetin B, isolated and efficiently purified from that mixture, is the main component and can be used as evidence. Solid phase extraction (SPE) proved to be an effective sample preparation method. Digoxin-d3 was used as the internal standard. Although ion suppression occurs, the limit of detection (LOD) is 0.27 ng/ml serum for thevetin B. Recovery is higher than 94%, and accuracy and precision were proficient. Method refinement was carried out with regard to developing a general screening method for cardiac glycosides. The assay is linear over the range of 0.5–8 ng/ml serum. Finally, the method was applied to a case of thevetia seed ingestion.

Item Type:	Article
Uncontrolled Keywords:	Thevetin B,Cardiac glycosides,Human serum,SPE,HPLC–ESI+-MS/MS Method validation
Subjects:	Science and mathematics Science and mathematics > Chemistry Science and mathematics > Plants (botany) Technology, medicine, applied sciences > Medicine and health
Divisions:	Faculty of Mathematics and Science > Institute for Chemistry and Biology of the Marine Environment (ICBM)
Date Deposited:	28 Feb 2014 11:47
Last Modified:	28 Feb 2014 11:47
URI:	https://oops.uni-oldenburg.de/id/eprint/1793
URN:	urn:nbn:de:gbv:715-oops-18746
DOI:	doi:10.1016/j.forsciint.2011.02.013
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