Lünemann, Daniel C. and Thomas, Anitta Rose and Xu, Jingjing and Bartölke, Rabea and Mouritsen, Henrik and De Sio, Antonietta and Lienau, Christoph (2021) Distinguishing between coherent and incoherent signals in excitation-emission spectroscopy. Optics express, 29 (15). pp. 24326-24337. ISSN 1094-4087

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Official URL: https://opg.optica.org/oe/fulltext.cfm?uri=oe-29-1...


The separation of incoherent emission signals from coherent light scattering often poses a challenge in (time-resolved) microscopy or excitation-emission spectroscopy. While in spectro-microscopy with narrowband excitation this is commonly overcome using spectral filtering, it is less straightforward when using broadband Fourier-transform techniques that are now becoming commonplace in, e.g., single molecule or ultrafast nonlinear spectroscopy. Here we show that such a separation is readily achieved using highly stable common-path interferometers for both excitation and detection. The approach is demonstrated for suppression of scattering from flavin adenine dinucleotide (FAD) and weakly emissive cryptochrome 4 (Cry4) protein samples. We expect that the approach will be beneficial, e.g., for fluorescence lifetime or Raman-based imaging and spectroscopy of various samples, including single quantum emitters.

Item Type: Article
Subjects: Science and mathematics > Physics
Divisions: Faculty of Mathematics and Science > Institute of Physics (IfP)
Date Deposited: 16 Mar 2022 11:39
Last Modified: 16 Mar 2022 11:39
URI: https://oops.uni-oldenburg.de/id/eprint/5377
URN: urn:nbn:de:gbv:715-oops-54589
DOI: 10.1364/OE.428850

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