Simone Christine, Heyen and Scholz-Böttcher, Barbara M. and Rabus, Ralf and Wilkes, Heinz (2021) Release of carboxylic acids into the exometabolome during anaerobic growth of a denitrifying bacterium with single substrates or crude oil. Organic geochemistry, 154. p. 104179. ISSN 0146-6380

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Anaerobic biodegradation of hydrocarbons mainly related to petroleum can be observed in numerous natural and anthropogenically overprinted environments. While microbiological and chemical tracers are used to study the development of such habitats, not much is known about the impact of particular bacteria on the system. Therefore, we used Aromatoleum sp. HxN1, an n-alkane-utilizing betaproteobacterium capable of degrading crude oil constituents under strictly anoxic conditions, as a model organism to examine its potential to release carboxylic acid metabolites into the surrounding environment. We conducted cultivation experiments with pure substrates, as well as with crude oil, and demonstrated that intermediates of catabolic pathways were highly abundant in the exometabolome. Strikingly, the metabolites found in highest concentrations in the cell-free culture supernatant of strain HxN1 grown with crude oil were associated with the transformation of potentially toxic aromatic hydrocarbons, which do not support growth of this particular bacterium. Additionally, a deficiency in coenzyme A caused by a highly productive substrate transformation may induce the excretion of a surplus of metabolites in order to regain coenzyme A. Under natural conditions, i.e., in the presence of mixed communities, such transformation products may be further degraded by other organisms, ultimately leading to mineralization.

Item Type: Article
Uncontrolled Keywords: Aromatoleum sp. HxN1; Solid phase extraction; Gas chromatography; Mass spectrometry; Detoxification; Succinic acids; Anionic exchange; Coenzyme A depletion; Transformation; Carboxylic acid metabolites
Divisions: Faculty of Mathematics and Science > Institute for Chemistry and Biology of the Marine Environment (ICBM)
Date Deposited: 10 Feb 2022 12:11
Last Modified: 11 Feb 2022 13:07
URN: urn:nbn:de:gbv:715-oops-53815
DOI: 10.1016/j.orggeochem.2020.104179

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