Heyen, Simone Christine and Scholz-Böttcher, Barbara M. and Rabus, Ralf and Wilkes, Heinz (2020) Method development and validation for the quantification of organic acids in microbial samples using anionic exchange solid-phase extraction and gas chromatography-mass spectrometry. Analytical and bioanalytical chemistry, 412 (27). pp. 7491-7503. ISSN 1618-2642 - 1618-2650

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Abstract

Organic acids play a key role in central metabolic functions of organisms, are crucial for understanding regulatory processes and are ubiquitous inside the cell. Therefore, quantification of these compounds provides a valuable approach for studying dynamics of metabolic processes, in particular when the organism faces changing environmental conditions. However, the extraction and analysis of organic acids can be challenging and validated methods available in this field are limited. In this study,we developed a method for the extraction and quantification of organic acids from microbial samples based on solid-phase extraction on a strong anionic exchange cartridge and gas chromatographic-mass spectrometric analysis. Full method validation was conducted to determine quality parameters of the new method. Recoveries for 12 of the 15 aromatic and aliphatic acids were between 100 and 111% and detection limits between 3 and 272 ng/mL. The ranges for the regression coefficients and process standard deviations for these compound classes were 0.9874–0.9994 and 0.04–0.69 μg/mL, respectively. Limitations were encountered when targeting aliphatic acids with hydroxy, oxo or enol ester functions. Finally, we demonstrated the applicability of the method on cell extracts of the bacterium Escherichia coli and the dinoflagellate Prorocentrum minimum.

Item Type:	Article
Uncontrolled Keywords:	Calibration; Recovery; Stability; Limit of detection; Limit of quantification; Statistics
Subjects:	Science and mathematics > Chemistry Science and mathematics > Life sciences, biology
Divisions:	Faculty of Mathematics and Science > Institute for Chemistry and Biology of the Marine Environment (ICBM)
Date Deposited:	10 Feb 2022 12:14
Last Modified:	11 Feb 2022 13:08
URI:	https://oops.uni-oldenburg.de/id/eprint/5299
URN:	urn:nbn:de:gbv:715-oops-53802
DOI:	10.1007/s00216-020-02883-3
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