Clemen, Ramona and Freund, Eric and Mrochen, Daniel and Miebach, Lea and Schmidt, Anke and Rauch, Bernhard H. and andere, (2021) Gas Plasma Technology Augments Ovalbumin Immunogenicity and OT-II T Cell Activation Conferring Tumor Protection in Mice. Advanced Science, 2021 (8).

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Reactive oxygen species (ROS/RNS) are produced during inflammation and elicit protein modifications, but the immunological consequences are largely unknown. Gas plasma technology capable of generating an unmatched variety of ROS/RNS is deployed to mimic inflammation and study the significance of ROS/RNS modifications using the model protein chicken ovalbumin (Ova vs oxOva). Dynamic light scattering and circular dichroism spectroscopy reveal structural modifications in oxOva compared to Ova. T cells from Ova-specific OT-II but not from C57BL/6 or SKH-1 wild type mice presents enhanced activation after Ova addition. OxOva exacerbates this activation when administered ex vivo or in vivo, along with an increased interferon-gamma production, a known anti-melanoma agent. OxOva vaccination of wild type mice followed by inoculation of syngeneic B16F10 Ova-expressing melanoma cells shows enhanced T cell number and activation, decreased tumor burden, and elevated numbers of antigen-presenting cells when compared to their Ova-vaccinated counterparts. Analysis of oxOva using mass spectrometry identifies three hot spots regions rich in oxidative modifications that are associated with the increased T cell activation. Using Ova as a model protein, the findings suggest an immunomodulating role of multi-ROS/RNS modifications that may spur novel research lines in inflammation research and for vaccination strategies in oncology.

Item Type: Article
Uncontrolled Keywords: kINPen, ovalbumin, oxPTM, ROS, vaccines
Divisions: Faculty of Medicine and Health Sciences > Department of Human Medicine
Date Deposited: 17 Jan 2022 10:21
Last Modified: 18 Jan 2022 07:14
URN: urn:nbn:de:gbv:715-oops-52666
DOI: 10.1002/advs.202003395

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