Siadjeu, Christian and Mayland-Quellhorst, Eike and Albach, Dirk Carl (2018) Genetic diversity and population structure of trifoliate yam (Dioscorea dumetorum Kunth) in Cameroon revealed by genotyping-by-sequencing (GBS). BMC Plant Biology, 18 (1). p. 359. ISSN 1471-2229

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Official URL: http://dx.doi.org/10.1186/s12870-018-1593-x

Abstract

Background: Yams (Dioscorea spp.) are economically important food for millions of people in the humid and subhumid tropics. Dioscorea dumetorum (Kunth) is the most nutritious among the eight-yam species, commonly grown and consumed in West and Central Africa. Despite these qualities, the storage ability of D. dumetorum is restricted by severe postharvest hardening of the tubers that can be addressed through concerted breeding efforts. The first step of any breeding program is bound to the study of genetic diversity. In this study, we used the Genotyping-By- Sequencing of Single Nucleotide Polymorphism (GBS-SNP) to investigate the genetic diversity and population structure of 44 accessions of D. dumetorum in Cameroon. Ploidy was inferred using flow cytometry and gbs2ploidy. Results: We obtained on average 6371 loci having at least information for 75% accessions. Based on 6457 unlinked SNPs, our results demonstrate that D. dumetorum is structured into four populations. We clearly identified, a western/ north-western, a western, and south-western populations, suggesting that altitude and farmers-consumers preference are the decisive factors for differential adaptation and separation of these populations. Bayesian and neighborjoining clustering detected the highest genetic variability in D. dumetorum accessions from the south-western region. This variation is likely due to larger breeding efforts in the region as shown by gene flow between D. dumetorum accessions from the south-western region inferred by maximum likelihood. Ploidy analysis revealed diploid and triploid levels in D. dumetorum accessions with mostly diploid accessions (77%). Male and female accessions were mostly triploid (75%) and diploid (69%), respectively. The 1C genome size values of D. dumetorum accessions were on average 0.333

Item Type: Article
Additional Information: Publiziert mit Hilfe des DFG-gef
Uncontrolled Keywords: Cameroon, D. dumetorum, Yam, Genetic diversity, Population structure, GBS, Ploidy
Subjects: Science and mathematics > Life sciences, biology
Science and mathematics > Plants (botany)
Divisions: Faculty of Mathematics and Science > Department of Biology and Environmental Sciences (IBU)
Date Deposited: 12 Sep 2019 11:13
Last Modified: 13 Sep 2019 09:54
URI: https://oops.uni-oldenburg.de/id/eprint/4163
URN: urn:nbn:de:gbv:715-oops-42448
DOI: doi:10.1186/s12870-018-1593-x
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