Kirchhoff, Christian and Ebert, Matthias and Jahn, Dieter and Cypionka, Heribert (2018) Chemiosmotic energy conservation in Dinoroseobacter shibae: proton translocation driven by aerobic respiration, denitrification, and photosynthetic light reaction. Frontiers in microbiology, 9. p. 903. ISSN 1664-302X

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Official URL: http://dx.doi.org/10.3389/fmicb.2018.00903

Abstract

Dinoroseobacter shibae is an aerobic anoxygenic phototroph and able to utilize light energy to support its aerobic energy metabolism. Since the cells can also grow anaerobically with nitrate and nitrite as terminal electron acceptor, we were interested in how the cells profit from photosynthesis during denitrification and what the steps of chemiosmotic energy conservation are. Therefore, we conducted proton translocation experiments and compared O2-, NO3-, and NO2- respiration during different light regimes and in the dark. We used wild type cells and transposon mutants with knocked-out nitrate- and nitrite- reductase genes (napA and nirS), as well as a mutant (ppsR) impaired in bacteriochlorophyll a synthesis. Light had a positive impact on proton translocation, independent of the type of terminal electron acceptor present. In the absence of an electron acceptor, however, light did not stimulate proton translocation. The light-driven add-on to proton translocation was about 1.4 H+/e- for O2 respiration and about 1.1 H+/e- for NO3- and NO2-. We could see that the chemiosmotic energy conservation during aerobic respiration involved proton translocation, mediated by the NADH dehydrogenase, the cytochrome bc1 complex, and the cytochrome c oxidase. During denitrification the last proton translocation step of the electron transport was missing, resulting in a lower H+/e- ratio during anoxia. Furthermore, we studied the type of light-harvesting and found that the cells were able to channel light from the green–blue spectrum most efficiently, while red light has only minor impact. This fits well with the depth profiles for D. shibae abundance in the ocean and the penetration depth of light with different wavelengths into the water column.

Item Type: Article
Additional Information: Publiziert mit Hilfe des DFG-geförderten Open Access-Publikationsfonds der Carl von Ossietzky Universität Oldenburg.
Uncontrolled Keywords: Roseobacter group, aerobic anoxygenic phototrophs, light-harvesting and channeling, napA, nirS
Subjects: Science and mathematics > Life sciences, biology
Divisions: Faculty of Mathematics and Science > Institute for Chemistry and Biology of the Marine Environment (ICBM)
Date Deposited: 11 Sep 2019 13:47
Last Modified: 25 Sep 2019 09:51
URI: https://oops.uni-oldenburg.de/id/eprint/4146
URN: urn:nbn:de:gbv:715-oops-42275
DOI: doi:10.3389/fmicb.2018.00903
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