Wimberg, Hanna and Janssen-Bienhold, Ulrike and Koch, Karl-Wilhelm (2018) Control of the nucleotide cycle in photoreceptor cell extracts by retinal degeneration protein 3. Frontiers in molecular neuroscience, 11. p. 52. ISSN 1662-5099
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Abstract
Retinal degeneration protein 3 (RD3) is crucial for photoreceptor cell survival and linked to Leber Congenital Amaurosis type 12 (LCA12), a hereditary retinal disease in humans. RD3 inhibits photoreceptor guanylate cyclases GC-E and GC-F and is involved in transport of GCs from the inner to the outer segments. Otherwise, its role in photoreceptor physiology is poorly understood. Here, we describe a new function of RD3. Purified RD3 evoked an increase in guanylate kinase activity, an enzyme that is involved in the nucleotide cycle in photoreceptors. We demonstrate a direct interaction between guanylate kinase and RD3 using back-scattering interferometry and show by immunohistochemistry of mouse retina sections that RD3 and guanylate kinase co-localize in photoreceptor inner segments and to a lesser extent in the outer plexiform layer. Our findings point toward a more complex function of RD3 in photoreceptors. The RD3 – guanylate kinase interaction may also play a role in other cellular systems, while the GC – RD3 interaction is exclusive to photoreceptors.
| Item Type: | Article |
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| Additional Information: | Publiziert mit Hilfe des DFG-geförderten Open Access-Publikationsfonds der Carl von Ossietzky Universität Oldenburg. |
| Uncontrolled Keywords: | RD3 protein, guanylate kinase, retinal dystrophy, cyclic nucleotide, phototransduction |
| Subjects: | Science and mathematics > Chemistry Science and mathematics > Life sciences, biology Technology, medicine, applied sciences > Medicine and health |
| Divisions: | Faculty of Medicine and Health Sciences > Department of Neuro Sciences |
| Date Deposited: | 10 Sep 2019 09:12 |
| Last Modified: | 12 Sep 2019 11:39 |
| URI: | https://oops.uni-oldenburg.de/id/eprint/4125 |
| URN: | urn:nbn:de:gbv:715-oops-42068 |
| DOI: | doi:10.3389/fnmol.2018.00052 |
| Nutzungslizenz: |
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