Mayland-Quellhorst, Eike and Meudt, Heidi M. and Albach, Dirk C. (2016) Transcriptomic Resources and Marker Validation for Diploid and Polyploid Veronica (Plantaginaceae) from New Zealand and Europe. Applications in Plant Sciences, 4 (10). p. 1600091. ISSN 2168-0450

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Premise of the study: Polyploidy may generate novel variation, leading to adaptation and species diversification. An excellent natural system to study polyploid evolution in a comparative framework is Veronica (Plantaginaceae), which comprises several parallel, recently evolved polyploid series. Methods: Over 105 million Illumina paired-end sequence reads were generated from cDNA libraries of leaf tissue from eight individuals representing three European and four New Zealand species. Forty-eight simple sequence repeat (SSR) and 48 low-copy nuclear (LCN) markers were developed and validated with Fluidigm microfluidic PCR and Illumina MiSeq amplicon sequencing on 48 different individuals each. Results: Individual Trinity assemblies were similar regarding annotated transcripts (13,009–14,271), mean contig length (635–742 bp), N50 value (916–1133 bp), E90N50 value (1099–1308 bp), contigs with positive BLAST hits (42–63%), and gene ontology terms. Analyses of 29,738 single-nucleotide polymorphisms (8746 phylogenetically informative) mined from these transcriptomes plus two outgroups (Picrorhiza kurrooa and Plantago ovata) showed moderate to high bootstrap support for all branches and reticulation among sampled European Veronica. Discussion: The transcriptome sequences themselves, as well as the validated SSR (40/48) and LCN (11/48) markers derived from them, show inter- and intraspecific genetic variation. These resources will be invaluable for future population genetic, phylogenetic, and functional genetic investigations in polyploid Veronica.

Item Type: Article
Additional Information: Publiziert mit Hilfe des DFG-geförderten Open Access-Publikationsfonds der Carl von Ossietzky Universität Oldenburg.
Uncontrolled Keywords: low-copy nuclear (LCN) markers, polyploidy, simple sequence repeat (SSR) markers, single-nucleotide polymorphisms (SNPs), transcriptome, Veronica
Subjects: Science and mathematics > Plants (botany)
Divisions: Faculty of Mathematics and Science > Department of Biology and Environmental Sciences (IBU)
Date Deposited: 20 Sep 2017 07:48
Last Modified: 20 Sep 2017 08:32
URN: urn:nbn:de:gbv:715-oops-33488
DOI: doi:10.3732/apps.1600091

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