Brüggen, Bianca and Kremser, Christiane and Bickert, Andreas and Ebel, Philipp and vom Dorp, Katharina and Schultz, Konrad and Dörmann, Peter and Willecke, Klaus and Dedek, Karin (2016) Defective ceramide synthases in mice cause reduced amplitudes in electroretinograms and altered sphingolipid composition in retina and cornea. European journal of neuroscience, 44 (1). pp. 1700-1713. ISSN 0953-816X

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Complex sphingolipids are strongly expressed in neuronal tissue and contain ceramides in their backbone. Ceramides are synthesized by six ceramide synthases (CerS1–6). Although it is known that each tissue has a unique profile of ceramide synthase expression and ceramide synthases are implicated in several neurodegenerative disorders, the expression of ceramide synthase isoforms has not been investigated in the retina. Here we demonstrate CerS1, CerS2 and CerS4 expression in mouse retina and cornea, with CerS4 ubiquitously expressed in all retinal neurons and Müller cells. To test whether ceramide synthase deficiency affects retinal function, we compared electroretinograms and retina morphology between wild-type and CerS1-, CerS2- and CerS4-deficient mice. Electroretinograms were strongly reduced in amplitude in ceramide synthase-deficient mice, suggesting that signalling in the outer retina is affected. However, the number of photoreceptors and cone outer segment length were unaltered and no changes in retinal layer thickness or synaptic structures were found. Mass spectrometric analyses of ceramides, hexosylceramides and sphingomyelins showed that C20 to C24 acyl-containing species were decreased whereas 16-containing species were increased in the retina of ceramide synthase-deficient mice. Similar but smaller changes were also found in the cornea. Thus, we hypothesize that the replacement of very long-chain fatty acyl residues by shorter C16 residues may affect the electricalproperties of retina and cornea, and alter receptor-mediated signal transduction, vesicle-mediated synaptic transmission or corneal light transmission. Future studies need to identify the molecular targets of ceramides or derived sphingolipids in light signal transduction and transmission in the eye.

Item Type: Article
Subjects: Science and mathematics > Life sciences, biology
Divisions: Faculty of Mathematics and Science > Department of Biology and Environmental Sciences (IBU)
Date Deposited: 12 Jan 2017 10:25
Last Modified: 13 Jan 2017 09:59
URN: urn:nbn:de:gbv:715-oops-29624
DOI: 10.1111/ejn.13260

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