Jarling, René and Kühner, Simon and Basílio Janke, Eline and Gruner, Andrea and Drozdowska, Marta and Golding, Bernard T. and Rabus, Ralf and Wilkes, Heinz (2015) Versatile transformations of hydrocarbons in anaerobic bacteria : substrate ranges and regio- and stereochemistry of activation reactions. Frontiers in microbiology, 6 (880). pp. 1-14. ISSN 1664-302X

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Abstract

Anaerobic metabolism of hydrocarbons proceeds either via addition to fumarate or by hydroxylation in various microorganisms, e.g., sulfate-reducing or denitrifying bacteria, which are specialized in utilizing n-alkanes or alkylbenzenes as growth substrates. General pathways for carbon assimilation and energy gain have been elucidated for a limited number of possible substrates. In this work the metabolic activity of 11 bacterial strains during anaerobic growth with crude oil was investigated and compared with the metabolite patterns appearing during anaerobic growth with more than 40 different hydrocarbons supplied as binary mixtures. We show that the range of co-metabolically formed alkyl- and arylalkyl-succinates is much broader in n-alkane than in alkylbenzene utilizers. The structures and stereochemistry of these products are resolved. Furthermore, we demonstrate that anaerobic hydroxylation of alkylbenzenes does not only occur in denitrifiers but also in sulfate reducers. We propose that these processes play a role in detoxification under conditions of solvent stress. The thermophilic sulfate-reducing strain TD3 is shown to produce n-alkylsuccinates, which are suggested not to derive from terminal activation of n-alkanes, but rather to represent intermediates of a metabolic pathway short-cutting fumarate regeneration by reverse action of succinate synthase. The outcomes of this study provide a basis for geochemically tracing such processes in natural habitats and contribute to an improved understanding of microbial activity in hydrocarbon-rich anoxic environments.

Item Type: Article
Uncontrolled Keywords: anaerobic metabolism hydrocarbons activation reactions n-alkylsuccinates detoxification
Subjects: Science and mathematics > Science
Science and mathematics > Chemistry
Science and mathematics > Earth sciences and geology
Science and mathematics > Life sciences, biology
Divisions: Faculty of Mathematics and Science > Institute for Chemistry and Biology of the Marine Environment (ICBM)
Date Deposited: 16 Feb 2016 11:24
Last Modified: 16 Feb 2016 11:24
URI: https://oops.uni-oldenburg.de/id/eprint/2671
URN: urn:nbn:de:gbv:715-oops-27522
DOI: 10.3389/fmicb.2015.00880
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